anti tom20 (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
anti tom20
Anti Tom20, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 255 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tom20/BNIP3L%2FNix+Rabbit+mAb/pmc13049664-189-26-27
Average 96 stars, based on 255 article reviews
Anti Tom20, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 255 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tom20/BNIP3L%2FNix+Rabbit+mAb/pmc13049664-189-26-27
Average 96 stars, based on 255 article reviews
anti tom20 - by Bioz Stars,
2026-10
96/100 stars
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Incubation:Article Title: COX5A induces M2 macrophage polarization in chronic rhinosinusitis with nasal polyps through ROS generation Article Snippet: For HE staining, the tissues were stained with the commercial HE kit (Beyotime, C0105M) according to the manufacturer’s instructions. .. After boiling in a citric acid buffer (Servicebio, G1202) for antigen recovery, the tissue sections were blocked with 5% Bovine Serum Albumin (BSA) (Beyotime, P0023B) in TBST (Servicebio, G0001) and then incubated with primary antibodies against CD206(1:400,Cell Signaling Technology (CST), 24595), COX5A (1:500, ABclonal, A6437), and Article Title: Blockade of T-type Ca2+ channels disrupts mitochondrial function and follicle development by inhibiting the PI3K/AKT pathway. Article Snippet: Calcium (Ca2+) function as critical secondary messengers in cellular signaling, essential for maintaining ovarian function and promoting oocyte maturation.. However, the contribution of T-type voltage-gated calcium channels (VGCCs) to ovarian follicle development remains unclear.. To investigate this, we administered flunarizine (FNZ), a potent T-type VGCCs antagonist, to 4-6-week-old female mice via intraperitoneal injection at doses of 0, 3, or 30 mg/kg/day for 7 days. Article Title: Onconase Induces Apoptosis in Dabrafenib-Resistant Melanoma Cell Lines Through Dysregulation of ROS Homeostasis, Antioxidant Protein Expression, and Mitochondrial Dynamics. Article Snippet: Protein extracts were separated by SDS–PAGE using 8.5–14% polyacrylamide gels, and subsequently transferred onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Milan, Italy), blocked for 1 h at RT in TBST buffer (10 mM Tris-HCl, pH 7.5; 100 mM NaCl; 0.1% Tween 20) containing 5% non-fat milk. .. Membranes were then incubated overnight at 4 ◦C with gentle agitation in 5% bovine serum albumin (BSA) containing the following primary antibodies: FTH1 (A19544); p(Ser40)-NRF2 (AP1133); HO-1 (A19062); GCLM (A11444); SLC7A11 (A2413); GSTP1 (A19061); FSP1 (A22278); cleaved PARP (A19612); LC3B (AA19665); p(T161)CDK1 (AP0324); p(Thr160)CDK2 (AP1364); p(Thr172)CDK4 (AP1364); p(ser616)-DRP1 (AP1353); FIS1 (A19666); PINK1 (A24745); ATPB (A11214); pERK1/2 (AP0974); ERK1/2 (A16686); UQCRC1 (A26343); (Abclonal, Woburn, MA, USA); ATG3 (GTX128065); Beclin1 (GTX133555); SOD2 (GTX630559); Glutathione reductase (GTX114199); COX4 (GTX114330); c-Myc (GTX103436); (GeneTex, Irvine, CA, USA); Mitofusin-1 (#14739); Mitofusin-2 (#11925); DRP1 (#8570); OPA1 (#80471); Article Title: COX5A induces M2 macrophage polarization in chronic rhinosinusitis with nasal polyps through ROS generation. Article Snippet: For HE staining, the tissues were stained with the commercial HE kit (Beyotime, C0105M) according to the manufacturer’s instructions. .. Immunofluorescence(mIF) After boiling in a citric acid buffer (Servicebio, G1202) for antigen recovery, the tissue sections were blocked with 5% Bovine Serum Albumin (BSA) (Beyotime, P0023B) in TBST (Servicebio, G0001) and then incubated with primary antibodies against CD206(1:400,Cell Signaling Technology (CST), 24595), COX5A (1:500, ABclonal, A6437), and Western Blot:Article Title: Parkinson's disease-associated PLA2G6 protects IP3R1 protein to control ER-mitochondria tethering and Ca 2+ transfer. Article Snippet: Immunoblotting signals were detected using a chemiluminescence reagent (Invitrogen, 32106) and quantified via ImageJ. .. The antibodies used for immunoblotting included PLA2G6 (Abcam, ab259950, 1:1000), GRP75 (Santa Cruz, sc-133137, 1:500), VDAC1 (Proteintech, 55259-1-AP, 1:1000), Sigma1R (CST, 61994, 1:1000), COX IV (ABclonal, A11631, 1:1000), Calreticulin (ABclonal, A20986, 1:1000), IP3R1 (ABclonal, A21471, 1:1000), MFN2 (Abcam, ab124773, 1:1000), TOM40 (ABclonal, A3213, 1:1000), Article Title: ER-localized ceramide accumulation contributes to replicative senescence. Article Snippet: Article ER-localized ceramide accumulation contributes to replicative senescence Ubiquitin Proteomics:Article Title: Parkinson's disease-associated PLA2G6 protects IP3R1 protein to control ER-mitochondria tethering and Ca 2+ transfer. Article Snippet: Immunoblotting signals were detected using a chemiluminescence reagent (Invitrogen, 32106) and quantified via ImageJ. .. The antibodies used for immunoblotting included PLA2G6 (Abcam, ab259950, 1:1000), GRP75 (Santa Cruz, sc-133137, 1:500), VDAC1 (Proteintech, 55259-1-AP, 1:1000), Sigma1R (CST, 61994, 1:1000), COX IV (ABclonal, A11631, 1:1000), Calreticulin (ABclonal, A20986, 1:1000), IP3R1 (ABclonal, A21471, 1:1000), MFN2 (Abcam, ab124773, 1:1000), TOM40 (ABclonal, A3213, 1:1000), Article Title: USP30 alleviates intestinal ischemia-reperfusion injury by deubiquitinating MFN2 mediating the mitochondrial endoplasmic reticulum pathway. Article Snippet: Intestinal ischemia-reperfusion (IIR) injury can cause intestinal barrier damage, systemic inflammatory response, and high mortality.. The key mechanism is the disorder of the mitochondrial-endoplasmic reticulum network.. Ubiquitin-specific peptidase 30 (USP30), located on the outer mitochondrial membrane, can reverse the partial ubiquitination of Parkin substrates or completely remove the ubiquitin chain to maintain mitochondrial function. Membrane:Article Title: Blockade of T-type Ca2+ channels disrupts mitochondrial function and follicle development by inhibiting the PI3K/AKT pathway. Article Snippet: Calcium (Ca2+) function as critical secondary messengers in cellular signaling, essential for maintaining ovarian function and promoting oocyte maturation.. However, the contribution of T-type voltage-gated calcium channels (VGCCs) to ovarian follicle development remains unclear.. To investigate this, we administered flunarizine (FNZ), a potent T-type VGCCs antagonist, to 4-6-week-old female mice via intraperitoneal injection at doses of 0, 3, or 30 mg/kg/day for 7 days. Gentle:Article Title: Onconase Induces Apoptosis in Dabrafenib-Resistant Melanoma Cell Lines Through Dysregulation of ROS Homeostasis, Antioxidant Protein Expression, and Mitochondrial Dynamics. Article Snippet: Protein extracts were separated by SDS–PAGE using 8.5–14% polyacrylamide gels, and subsequently transferred onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Milan, Italy), blocked for 1 h at RT in TBST buffer (10 mM Tris-HCl, pH 7.5; 100 mM NaCl; 0.1% Tween 20) containing 5% non-fat milk. .. Membranes were then incubated overnight at 4 ◦C with gentle agitation in 5% bovine serum albumin (BSA) containing the following primary antibodies: FTH1 (A19544); p(Ser40)-NRF2 (AP1133); HO-1 (A19062); GCLM (A11444); SLC7A11 (A2413); GSTP1 (A19061); FSP1 (A22278); cleaved PARP (A19612); LC3B (AA19665); p(T161)CDK1 (AP0324); p(Thr160)CDK2 (AP1364); p(Thr172)CDK4 (AP1364); p(ser616)-DRP1 (AP1353); FIS1 (A19666); PINK1 (A24745); ATPB (A11214); pERK1/2 (AP0974); ERK1/2 (A16686); UQCRC1 (A26343); (Abclonal, Woburn, MA, USA); ATG3 (GTX128065); Beclin1 (GTX133555); SOD2 (GTX630559); Glutathione reductase (GTX114199); COX4 (GTX114330); c-Myc (GTX103436); (GeneTex, Irvine, CA, USA); Mitofusin-1 (#14739); Mitofusin-2 (#11925); DRP1 (#8570); OPA1 (#80471); Immunofluorescence:Article Title: COX5A induces M2 macrophage polarization in chronic rhinosinusitis with nasal polyps through ROS generation. Article Snippet: For HE staining, the tissues were stained with the commercial HE kit (Beyotime, C0105M) according to the manufacturer’s instructions. .. Immunofluorescence(mIF) After boiling in a citric acid buffer (Servicebio, G1202) for antigen recovery, the tissue sections were blocked with 5% Bovine Serum Albumin (BSA) (Beyotime, P0023B) in TBST (Servicebio, G0001) and then incubated with primary antibodies against CD206(1:400,Cell Signaling Technology (CST), 24595), COX5A (1:500, ABclonal, A6437), and |
